Share Email Print

Proceedings Paper

Linking cell shape, elasticity and fate: in vitro re-differentiation of chondrocytes
Author(s): Xiaofei Yuan; Yahua Chim; Huabing Yin
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Autologous chondrocyte transplantation (ACT) has become a promising method for repairing large articular defects. However, dedifferentiation of chondrocytes during cell expansion remains a major limitation for ACT procedures. In this study, we explore the potential of confining cell shape for re-differentiation of dedifferentiated bovine chondrocytes. A novel culture system, combining 2D micropatterning with 3D matrix formation, was developed to control and maintain individual chondrocyte’s shape. Both collagen II synthesis and the mechanical properties of cells were monitored during re-differentiation. We show that a spherical morphology without cell spreading plays a limited role in induction of re-differentiation. Instead, isolated, dedifferentiated chondrocytes partially regain chondrogenic properties if they have an appropriate cell shape and limited spreading.

Paper Details

Date Published: 26 February 2014
PDF: 7 pages
Proc. SPIE 8946, Optical Elastography and Tissue Biomechanics, 89460M (26 February 2014); doi: 10.1117/12.2039859
Show Author Affiliations
Xiaofei Yuan, Univ. of Glasgow (United Kingdom)
Yahua Chim, Univ. of Glasgow (United Kingdom)
Huabing Yin, Univ. of Glasgow (United Kingdom)

Published in SPIE Proceedings Vol. 8946:
Optical Elastography and Tissue Biomechanics
Kirill V. Larin; David D. Sampson, Editor(s)

© SPIE. Terms of Use
Back to Top