Share Email Print
cover

Proceedings Paper

IgG/anti-IgG immunoassay based on a turn-around point long period grating
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Long period fiber gratings (LPFGs) have been proposed as label-free optical biosensor for a few years. Refractive index changes, which modify the fiber transmission spectrum, are still used for evaluating a biochemical interaction that occurs along the grating region. A turn-around point (TAP) LPFG was manufactured for enhancing the refractive index sensitivity of these devices. Considering the simplicity and the fast process with respect to the silanization procedure, the functionalization of the fiber was carried out by Eudragit L100 copolymer. An IgG/anti-IgG immunoassay was implemented for studying the antigen/antibody interaction. A limit of detection lower than 100 μg L-1 was achieved. Based on the same model assay, we compared the resonance wavelength shifts during the injection of 10 mg L-1 anti-IgG antigen between the TAP LPFG and a standard non-TAP one, in which the coupling occurs with a lower order cladding mode, as performance improvement of the LPFG-based biosensors.

Paper Details

Date Published: 27 February 2014
PDF: 6 pages
Proc. SPIE 8935, Advanced Biomedical and Clinical Diagnostic Systems XII, 89350V (27 February 2014); doi: 10.1117/12.2039782
Show Author Affiliations
F. Chiavaioli, IFAC-CNR, Istituto di Fisica Applicata Nello Carrara (Italy)
P. Biswas, Central Glass and Ceramic Research Institute (India)
C. Trono, IFAC-CNR, Istituto di Fisica Applicata Nello Carrara (Italy)
A. Giannetti, IFAC-CNR, Istituto di Fisica Applicata Nello Carrara (Italy)
S. Tombelli, IFAC-CNR, Istituto di Fisica Applicata Nello Carrara (Italy)
S. Bandyopadhyay, Central Glass and Ceramic Research Institute (India)
N. Basumallick, Central Glass and Ceramic Research Institute (India)
K. Dasgupta, Central Glass and Ceramic Research Institute (India)
F. Baldini, IFAC-CNR, Istituto di Fisica Applicata Nello Carrara (Italy)


Published in SPIE Proceedings Vol. 8935:
Advanced Biomedical and Clinical Diagnostic Systems XII
Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest, Editor(s)

© SPIE. Terms of Use
Back to Top