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Proceedings Paper

Fiber bundle based endomicroscopy prototype with two collection channels for simultaneous coherent anti-Stokes Raman scattering and second harmonic generation imaging
Author(s): Zhengfan Liu; Zachary A. Satira; Xi Wang; Xiaoyun Xu; Xu Chen; Kelvin Wong; Shufen Chen; Jianguo Xin; Stephen T. C. Wong
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Paper Abstract

Label-free multiphoton imaging is promising for replacing biopsy and could offer new strategies for intraoperative or surgical applications. Coherent anti-Stokes Raman scattering (CARS) imaging could provide lipid-band contrast, and second harmonic generation (SHG) imaging is useful for imaging collagen, tendon and muscle fibers. A combination of these two imaging modalities could provide rich information and this combination has been studied by researchers to investigate diseases through microscopy imaging. The combination of these two imaging modalities in endomicroscopy imaging has been rarely investigated. In this research, a fiber bundle consisted of one excitation fiber and 18 collection fibers was developed in our endomicroscopy prototype. The 18 collection fibers were divided into two collection channels with 9 fibers in each channel. These two channels could be used together as one channel for effective signal collection or used separately for simplifying detection part of the system. Differences of collection pattern of these two channels were investigated. Collection difference of central excitation fiber and surrounding 18 fibers was also investigated, which reveals the potential ability of this system to measure forward to backward (F/B) ratio in SHG imaging. CARS imaging of mouse adipocyte and SHG imaging of mouse tail tendon were performed to demonstrate the CARS and SHG tissue imaging performance of this system. Simultaneous CARS and SHG imaging ability of this system was demonstrated by mouse tail imaging. This fiber bundle based endomicroscopy imaging prototype, offers a promising platform for constructing efficient fiber-based CARS and SHG multimodal endomicroscopes for label free intraoperative imaging applications.

Paper Details

Date Published: 28 February 2014
PDF: 9 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894814 (28 February 2014); doi: 10.1117/12.2039559
Show Author Affiliations
Zhengfan Liu, Beijing Institute of Technology (China)
Weill Cornell Medical College (United States)
Zachary A. Satira, Weill Cornell Medical College (United States)
Rice Univ. (United States)
Xi Wang, Weill Cornell Medical College (United States)
Xiaoyun Xu, Weill Cornell Medical College (United States)
Xu Chen, Weill Cornell Medical College (United States)
Kelvin Wong, Weill Cornell Medical College (United States)
Shufen Chen, Beijing Institute of Technology (China)
Jianguo Xin, Beijing Institute of Technology (China)
Stephen T. C. Wong, Weill Cornell Medical College (United States)
Rice Univ. (United States)


Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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