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Proceedings Paper

3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy
Author(s): F. Merola; L. Miccio; P. Memmolo; G. Di Caprio; G. Coppola; P. Netti; P. Ferraro
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Paper Abstract

We present the possibility to trap cells (mouse fibroblasts, bovine spermatozoa and diatoms), to manage their position and to induce rotation, by using optical tweezers. The aim is to place them in desired positions, in order to record holographic images in a microscope configuration. Then we are able to recover the 3D shape and to calculate the biovolume of the cells starting from the reconstructed quantitative phase maps (QPMs).

Paper Details

Date Published: 4 March 2014
PDF: 8 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471A (4 March 2014); doi: 10.1117/12.2039423
Show Author Affiliations
F. Merola, Istituto Nazionale di Ottica, CNR (Italy)
L. Miccio, Istituto Nazionale di Ottica, CNR (Italy)
P. Memmolo, Istituto Nazionale di Ottica, CNR (Italy)
Istituto Italiano di Tecnologia (Italy)
G. Di Caprio, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
The Rowland Institute at Harvard, Harvard Univ. (United States)
G. Coppola, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
P. Netti, Istituto Italiano di Tecnologia (Italy)
P. Ferraro, Istituto Nazionale di Ottica, CNR (Italy)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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