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Proceedings Paper

Two-photon in vivo imaging of retinal microstructures
Author(s): Adi Schejter; Nairouz Farah; Shy Shoham
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Paper Abstract

Non-invasive fluorescence retinal imaging in small animals is an important requirement in an array of translational vision applications. Two-photon imaging has the potential for long-term investigation of healthy and diseased retinal function and structure in vivo. Here, we demonstrate that two-photon microscopy through a mouse’s pupil can yield high-quality optically sectioned fundus images. By remotely scanning using an electronically tunable lens we acquire highly-resolved 3D fluorescein angiograms. These results provide an important step towards various applications that will benefit from the use of infrared light, including functional imaging of retinal responses to light stimulation.

Paper Details

Date Published: 28 February 2014
PDF: 5 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894824 (28 February 2014); doi: 10.1117/12.2039375
Show Author Affiliations
Adi Schejter, Technion-Israel Institute of Technology (Israel)
Nairouz Farah, Technion-Israel Institute of Technology (Israel)
Shy Shoham, Technion-Israel Institute of Technology (Israel)

Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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