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Proceedings Paper

Photodynamic inactivation of microorganisms which cause pulmonary diseases with infrared light: an in vitro study
Author(s): Ilaiáli Souza Leite; Mariana C. Geralde; Ana C. Salina; Alexandra I. Medeiros; Cristina Kurachi; Vanderlei S. Bagnato; Natalia M. Inada
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Paper Abstract

Lower respiratory infections are among the leading causes of death worldwide. In this study, it was evaluated the interaction of indocyanine green, a photosensitizer activated by infrared light, with alveolar macrophages and the effectiveness of the photodynamic therapy using this compound against Streptococcus pneumoniae . Initial experiments analyzed indocyanine green toxicity to alveolar macrophages in the dark with different drug concentrations and incubation times, and macrophage viability was obtained with the MTT method. The average of the results showed viability values below 90% for the two highest concentrations. Experiments with Streptococcus pneumoniae showed photodynamic inactivation with 10 μM indocyanine green solution. Further experiments with the bacteria in co-culture with AM will be conducted verifying the photodynamic inactivation effectiveness of the tested drug concentrations and incubation periods using infrared light.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8927, Endoscopic Microscopy IX; and Optical Techniques in Pulmonary Medicine, 89271A (4 March 2014); doi: 10.1117/12.2039300
Show Author Affiliations
Ilaiáli Souza Leite, Univ. de São Paulo (Brazil)
Mariana C. Geralde, Univ. Federal de São Carlos (Brazil)
Ana C. Salina, Univ. Estadual Paulista (Brazil)
Alexandra I. Medeiros, Univ. Estadual Paulista (Brazil)
Cristina Kurachi, Univ. de São Paulo (Brazil)
Vanderlei S. Bagnato, Univ. de São Paulo (Brazil)
Natalia M. Inada, Univ. de São Paulo (Brazil)

Published in SPIE Proceedings Vol. 8927:
Endoscopic Microscopy IX; and Optical Techniques in Pulmonary Medicine
Melissa J. Suter; Guillermo J. Tearney; Thomas D. Wang; Stephen Lam; Matthew Brenner, Editor(s)

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