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Proceedings Paper

Fluorescence lifetime imaging microscopy using a streak camera
Author(s): Lixin Liu; Yahui Li; Luogeng Sun; Heng Li; Xiao Peng; Junle Qu
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Paper Abstract

We present the development of a fluorescence lifetime imaging microscopy system using a streak camera (SC-FLIM), which uses ultrafast infrared laser for multiphoton excitation and a streak camera for lifetime measurement. A pair of galvo mirrors are employed to accomplish quick time-resolved scanning on a line and 2D fluorescence lifetime imaging. The SC-FLIM system was calibrated using an F-P etalon and several standard fluorescent dyes, and was also used to perform fluorescence lifetime imaging of fluorescent microspheres and a prepared plant stem slide.

Paper Details

Date Published: 28 February 2014
PDF: 5 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482L (28 February 2014); doi: 10.1117/12.2039056
Show Author Affiliations
Lixin Liu, Xidian Univ. (China)
Yahui Li, Xidian Univ. (China)
Luogeng Sun, Xidian Univ. (China)
Heng Li, Shenzhen Univ. (China)
Xiao Peng, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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