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Proceedings Paper

Asymmetric-detection time-stretch optical microscopy (ATOM) for high-contrast and high-speed microfluidic cellular imaging
Author(s): Terence T. W. Wong; Andy K. S. Lau; Matthew Y. H. Tang; Kenneth K. Y. Ho; Kenneth K. Y. Wong; Anderson H. C. Shum; Kevin K. Tsia
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Paper Abstract

High-throughput cellular imaging is acclaimed as captivating yet challenging in biomedical diagnostics. We have demonstrated a new imaging modality, asymmetric-detection time-stretch optical microscopy (ATOM), by incorporating a simple detection scheme which is a further advancement in time-stretch microscopy – a viable solution to achieve high-speed and high-throughput cellular imaging. Through the asymmetric-detection scheme in ATOM, the time-stretch image contrast is enhanced through accessing to the phase-gradient information. With the operation in the 1 μm wavelength range, we demonstrate high-resolution and high-contrast cellular imaging in ultrafast microfluidic flow (up to 10 m/s) by ATOM – achieving an imaging throughput equivalent to ~100,000 cells/sec.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471D (4 March 2014); doi: 10.1117/12.2038952
Show Author Affiliations
Terence T. W. Wong, The Univ. of Hong Kong (Hong Kong, China)
Andy K. S. Lau, The Univ. of Hong Kong (Hong Kong, China)
Matthew Y. H. Tang, The Univ. of Hong Kong (Hong Kong, China)
Kenneth K. Y. Ho, The Univ. of Hong Kong (Hong Kong, China)
Kenneth K. Y. Wong, The Univ. of Hong Kong (Hong Kong, China)
Anderson H. C. Shum, The Univ. of Hong Kong (Hong Kong, China)
Kevin K. Tsia, The Univ. of Hong Kong (Hong Kong, China)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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