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Proceedings Paper

Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin
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Paper Abstract

Multiphoton fluorescence microscopy (MPM) is a method for high resolution, non-invasive investigations of biological tissue. The aim of introducing an annular shaped laser beam is to reduce the ouf-of-focus generated background signal improving imaging of light scattering tissue such as human skin. Simulations show that 50% of the beam radius can be blocked, while preserving the shape of the point spread function. Initial experiments performed on a phantom consisting of fluorescein and fluorescent beads embedded in agar by using a custom built MPM-set up show that by introducing a simple beam blocker to create an annular beam, the background signal is reduced with approximately 5%. Future work will include optimizing the set up, and creating phantoms with more light scattering properties.

Paper Details

Date Published: 28 February 2014
PDF: 11 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482K (28 February 2014); doi: 10.1117/12.2038942
Show Author Affiliations
Johan Borglin, Göteborgs Univ. (Sweden)
Nicholas J. Durr, Massachusetts Institute of Technology (United States)
Stina Guldbrand, Göteborgs Univ. (Sweden)
Onur Ferhanoglu, The Univ. of Texas at Austin (United States)
Ann-Marie Wennberg, Sahlgrenska Univ. Hospital (Sweden)
Dag Hanstorp, Göteborgs Univ. (Sweden)
Adela Ben-Yakar, The Univ. of Texas at Austin (United States)
Marica B. Ericson, Göteborgs Univ. (Sweden)

Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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