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Proceedings Paper

Enhanced angiogenic effect of adipose-derived stromal cell spheroid with low-level light therapy in hindlimb ischemia mice
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Paper Abstract

Adipose-derived stromal cells (ASCs) are attractive cell source for tissue engineering. However, one obstacle to this approach is that the transplanted ASC population can decline rapidly in the recipient tissue. The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on transplanted human ASCs (hASCs) spheroid in a hindlimb ischemia animal model. LLLT, hASCs spheroid and hASCs spheroid transplantation with LLLT (spheroid + LLLT) were applied to the ischemic hindlimbs in athymic mice. The survival, differentiation and secretion of vascular endothelial growth (VEGF) of spheroid ASCs were evaluated by immunohistochemistry. The spheroid + LLLT group enhanced the tissue regeneration, including angiogenesis, compared with other groups. The spheroid contributed tissue regeneration via differentiation and secretion of growth factors. In the spheroid + LLLT group, the survival of spheroid hASCs was increased by the decreased apoptosis of spheroid hASCs in the ischemic hindlimb. The secretion of growth factors was stimulated in the spheroid + LLLT group compared with the ASCs group and spheroid group. These data suggest that LLLT is an effective biostimulator of spheroid hASCs in tissue regeneration that enhances the survival of ASCs and stimulates the secretion of growth factors in the ischemic hindlimb.

Paper Details

Date Published: 18 February 2014
PDF: 7 pages
Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 893215 (18 February 2014); doi: 10.1117/12.2038904
Show Author Affiliations
In-Su Park, Beckman Laser Institute-Korea (Korea, Republic of)
Dankook Univ. (Korea, Republic of)
Jin-Chul Ahn, Beckman Laser Institute-Korea (Korea, Republic of)
Dankook Univ. (Korea, Republic of)
Phil-Sang Chung, Beckman Laser Institute-Korea (Korea, Republic of)
Dankook Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 8932:
Mechanisms for Low-Light Therapy IX
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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