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Proceedings Paper

Near-infrared diffuse reflectance imaging of infarct core and peri-infarct depolarization in a rat middle cerebral artery occlusion model
Author(s): Satoko Kawauchi; Izumi Nishidate; Hiroshi Nawashiro; Shunichi Sato
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Paper Abstract

To understand the pathophysiology of ischemic stroke, in vivo imaging of the brain tissue viability and related spreading depolarization is crucial. In the infarct core, impairment of energy metabolism causes anoxic depolarization (AD), which considerably increases energy consumption, accelerating irreversible neuronal damage. In the peri-infarct penumbra region, where tissue is still reversible despite limited blood flow, peri-infarct depolarization (PID) occurs, exacerbating energy deficit and hence expanding the infarct area. We previously showed that light-scattering signal, which is sensitive to cellular/subcellular structural integrity, was correlated with AD and brain tissue viability in a rat hypoxia-reoxygenation model. In the present study, we performed transcranial NIR diffuse reflectance imaging of the rat brain during middle cerebral artery (MCA) occlusion and examined whether the infarct core and PIDs can be detected. Immediately after occluding the left MCA, light scattering started to increase focally in the occlusion site and a bright region was generated near the occlusion site and spread over the left entire cortex, which was followed by a dark region, showing the occurrence of PID. The PID was generated repetitively and the number of times of occurrence in a rat ranged from four to ten within 1 hour after occlusion (n=4). The scattering increase in the occlusion site was irreversible and the area with increased scattering expanded with increasing the number of PIDs, indicating an expansion of the infarct core. These results suggest the usefulness of NIR diffuse reflectance signal to visualize spatiotemporal changes in the infarct area and PIDs.

Paper Details

Date Published: 5 March 2014
PDF: 6 pages
Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89280K (5 March 2014); doi: 10.1117/12.2038771
Show Author Affiliations
Satoko Kawauchi, National Defense Medical College (Japan)
Izumi Nishidate, Tokyo Univ. of Agriculture and Technology (Japan)
Hiroshi Nawashiro, Tokorozawa Central Hospital (Japan)
Shunichi Sato, National Defense Medical College (Japan)


Published in SPIE Proceedings Vol. 8928:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
Henry Hirschberg; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Steen J. Madsen, Editor(s)

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