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Proceedings Paper

Revisit laser scanning fluorescence microscopy performance under fluorescence-lifetime-limited regime
Author(s): Antony C. Chan; Terence T. W. Wong; Kenneth K. Y. Wong; Edmund Y. Lam; Kevin K. Tsia
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Paper Abstract

Continuing desire for higher-speed laser scanning fluorescence microscopy (LSFM) and progressive advancement in ultrafast and sensitive photodetectors might imply that our conventional understanding of LSFM is not adequate when approaching to the intrinsic speed limit — fluorescence lifetime. In this regard, we here revisit the theoretical framework of LSFM and evaluate its general performance in lifetime-limited and noise-limited regimes. Our model suggests that there still exists an order-of-magnitude gap between the current LSFM speed and the intrinsic limit. An imaging frame rate of > 100 kHz could be viable with the emerging laser-scanning techniques using ultrafast wavelength-swept sources, or optical time-stretch.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 894726 (4 March 2014); doi: 10.1117/12.2038766
Show Author Affiliations
Antony C. Chan, The Univ. of Hong Kong (Hong Kong, China)
Terence T. W. Wong, The Univ. of Hong Kong (Hong Kong, China)
Kenneth K. Y. Wong, The Univ. of Hong Kong (Hong Kong, China)
Edmund Y. Lam, The Univ. of Hong Kong (Hong Kong, China)
Kevin K. Tsia, The Univ. of Hong Kong (Hong Kong, China)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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