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Proceedings Paper

Fluorescent imaging over an ultra-large field-of-view of 532 cm2 using a flatbed scanner
Author(s): Zoltán S. Göröcs; Yuye Ling; Meng D. Yu; Dimitri Karahalios; Kian Mogharabi; Kenny Lu; Qingshan Wei; Aydogan Ozcan
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Paper Abstract

We introduce a fluorescent imaging method that is capable of detecting fluorescent micro-particles over an ultra-wide field of view of 19 cm × 28 cm using a modified flatbed scanner. We added a custom-designed absorbing emission filter, a computer controlled two dimensional LED array, and modified the driver of the scanner to maximize the sensitivity, exposure time, and gain for fluorescent detection of micro-objects. This high-throughput fluorescent imaging device used in conjunction with a microfluidic sample holder enables rapid screening of fluorescent micro-objects inside more than 2.2mL of optically dense media (i.e., whole blood) within 5 minutes. The device is sensitive enough to detect fluorescently labeled cells, and generates images that have an effective pixel count of 2.2 Giga-pixels.

Paper Details

Date Published: 12 March 2014
PDF: 8 pages
Proc. SPIE 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, 89510D (12 March 2014); doi: 10.1117/12.2038602
Show Author Affiliations
Zoltán S. Göröcs, Univ. of California, Los Angeles (United States)
Yuye Ling, Univ. of California, Los Angeles (United States)
Meng D. Yu, Univ. of California, Los Angeles (United States)
Dimitri Karahalios, Univ. of California, Los Angeles (United States)
Kian Mogharabi, Univ. of California, Los Angeles (United States)
Kenny Lu, Univ. of California, Los Angeles (United States)
Qingshan Wei, Univ. of California, Los Angeles (United States)
Aydogan Ozcan, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 8951:
Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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