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Proceedings Paper

Monitoring the influence of antibiotic exposure using Raman spectroscopy
Author(s): Ota Samek; Pavel Zemanek; Silvie Bernatova; Jan Jezek; Mojmir Sery; Petr Jakl; Martin Siler; Filip Ruzicka
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Paper Abstract

Here we report on combination of the data obtained from MICs (minimum inhibitory concentrations) with infor- mation of microoragnisms fingerprint provided by Raman spectroscopy. In our feasibility study we could follow mechanisms of the bacteriostatic versus bactericidal action on biofilm-positive Staphylococcus epidermidis simply by monitoring Raman bands corresponding to DNA translating the changes introduced by selected antibiotics. The Raman spectra of Staphylococcus epidermidis treated with a bacteriostatic agent show little effect on DNA which is in contrast with the action of a bactericidal agent where decreased in dedicated Raman spectra signal strength suggests DNA fragmentation. Moreover, we demonstrate that Raman tweezers are indeed able to distinguish strains of biofilm-forming (biofilm-positive) and biofilm-negative Staphylococcus epidermidis strains using principal component analysis (PCA).

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390P (4 March 2014); doi: 10.1117/12.2038595
Show Author Affiliations
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemanek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Silvie Bernatova, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Jezek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Mojmir Sery, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Petr Jakl, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Martin Siler, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Filip Ruzicka, Faculty of Medicine and St. Anne's Faculty Hospital (Czech Republic)


Published in SPIE Proceedings Vol. 8939:
Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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