Share Email Print
cover

Proceedings Paper

Intracellular temperature mapping with fluorescence-assisted photoacoustic thermometry
Author(s): Liang Gao; Chi Zhang; Chiye Li; Lihong Wang
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here we present a technique – fluorescence-assisted photoacoustic thermometry (FAPT) – for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 °C) temperature resolution and sub-micron (0.23 μm) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration- and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing.

Paper Details

Date Published: 3 March 2014
PDF: 5 pages
Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 894309 (3 March 2014); doi: 10.1117/12.2038569
Show Author Affiliations
Liang Gao, Washington Univ. in St. Louis (United States)
Chi Zhang, Washington Univ. in St. Louis (United States)
Chiye Li, Washington Univ. in St. Louis (United States)
Lihong Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 8943:
Photons Plus Ultrasound: Imaging and Sensing 2014
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

© SPIE. Terms of Use
Back to Top