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Proceedings Paper

Photothermal bleaching in time-lapse photoacoustic microscopy
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Paper Abstract

We studied the phenomenon of photothermal bleaching — a gradual reduction of contrast agent particles during repeated scans in photoacoustic microscopy. The dependence of the photothermal bleaching rate on the excitation pulse energy was determined while the laser focal diameter was held constant. Our results showed that, the dependence of the photothermal bleaching rate on the excitation pulse energy differed before and after the absorbers were raised to their melting point by the deposited laser energy. Based on this finding, we suggested an optimal excitation pulse energy, which balances the photothermal bleaching rate and signal amplitude, for time-lapse imaging applications.

Paper Details

Date Published: 3 March 2014
PDF: 7 pages
Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89433G (3 March 2014); doi: 10.1117/12.2038564
Show Author Affiliations
Liang Gao, Washington Univ. in St. Louis (United States)
Lidai Wang, Washington Univ. in St. Louis (United States)
Chiye Li, Washington Univ. in St. Louis (United States)
Alejandro Garcia-Uribe, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 8943:
Photons Plus Ultrasound: Imaging and Sensing 2014
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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