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Proceedings Paper

Multi-spectral scanning fiber endoscope with concurrent autofluorescence mitigation for enhanced target-to-background ratio imaging
Author(s): Chenying Yang; Vivian W. Hou; Leonard Y. Nelson; Richard S. Johnston; C. David Melville; Eric J. Seibel
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Paper Abstract

We developed a multispectral fluorescence-reflectance scanning fiber endoscope (SFE) for wide-field molecular imaging of fluorescence-labeled molecular probes. Concurrent multi-channels imaging with the wide-field SFE also allows for real-time mitigation of background autofluorescence (AF) signal, especially when the FDA approved fluorescein is used as the target fluorophore. In the current study, we demonstrated a real-time AF mitigation algorithm on a tissue phantom which featured molecular probe targeted cells of high grade dysplasia on a substrate containing AF species. The targetto- background ratio was enhanced by over an order of magnitude when applying the real-time AF mitigation algorithm. By minimizing the background signal, multispectral fluorescence imaging can provide sufficient image contrast and quantitative target information for detecting small pre-cancerous lesions in vivo.

Paper Details

Date Published: 4 March 2014
PDF: 9 pages
Proc. SPIE 8927, Endoscopic Microscopy IX; and Optical Techniques in Pulmonary Medicine, 89270I (4 March 2014); doi: 10.1117/12.2038553
Show Author Affiliations
Chenying Yang, Univ. of Washington (United States)
Vivian W. Hou, Univ. of Washington (United States)
Leonard Y. Nelson, Univ. of Washington (United States)
Richard S. Johnston, Univ. of Washington (United States)
C. David Melville, Univ. of Washington (United States)
Eric J. Seibel, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 8927:
Endoscopic Microscopy IX; and Optical Techniques in Pulmonary Medicine
Melissa J. Suter; Guillermo J. Tearney; Thomas D. Wang; Stephen Lam; Matthew Brenner, Editor(s)

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