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Proceedings Paper

3D reconstruction of internal structure of animal body using near-infrared light
Author(s): Trung Nghia Tran; Kohei Yamamoto; Takeshi Namita; Yuji Kato; Koichi Shimizu
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Paper Abstract

To realize three-dimensional (3D) optical imaging of the internal structure of animal body, we have developed a new technique to reconstruct CT images from two-dimensional (2D) transillumination images. In transillumination imaging, the image is blurred due to the strong scattering in the tissue. We had developed a scattering suppression technique using the point spread function (PSF) for a fluorescent light source in the body. In this study, we have newly proposed a technique to apply this PSF for a light source to the image of unknown light-absorbing structure. The effectiveness of the proposed technique was examined in the experiments with a model phantom and a mouse. In the phantom experiment, the absorbers were placed in the tissue-equivalent medium to simulate the light-absorbing organs in mouse body. Near-infrared light was illuminated from one side of the phantom and the image was recorded with CMOS camera from another side. Using the proposed techniques, the scattering effect was efficiently suppressed and the absorbing structure can be visualized in the 2D transillumination image. Using the 2D images obtained in many different orientations, we could reconstruct the 3D image. In the mouse experiment, an anesthetized mouse was held in an acrylic cylindrical holder. We can visualize the internal organs such as kidneys through mouse’s abdomen using the proposed technique. The 3D image of the kidneys and a part of the liver were reconstructed. Through these experimental studies, the feasibility of practical 3D imaging of the internal light-absorbing structure of a small animal was verified.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8952, Biomedical Applications of Light Scattering VIII, 89521A (4 March 2014); doi: 10.1117/12.2038474
Show Author Affiliations
Trung Nghia Tran, Hokkaido Univ. (Japan)
Kohei Yamamoto, Hokkaido Univ. (Japan)
Takeshi Namita, Kyoto Univ. (Japan)
Yuji Kato, Hokkaido Univ. (Japan)
Koichi Shimizu, Hokkaido Univ. (Japan)

Published in SPIE Proceedings Vol. 8952:
Biomedical Applications of Light Scattering VIII
Adam Wax; Vadim Backman, Editor(s)

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