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Proceedings Paper

Label-free molecular imaging
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Paper Abstract

Optical microscopy technology has achieved great improvements in the 20th century. The detection limit has reached about twenty nanometers (with near-field optics, STED, PALM and STORM). But in the application areas such as life science, medical science, clinical treatment and especially in vivo dynamic measurement, mutual restrictions still exist between numeric aperture/magnification and working distance, fluorescent dependent, and between resolution and frame rate/field size, etc. This paper explores a hyperspectral scanning super-resolution label free molecules imaging method based on the white light interferometry. The vertical detection resolution was approximate to 1 nm which is the thickness of a single molecular layer and dynamic measuring range of thickness reaches to 10 μm. The spectrum-shifting algorithm is developed for robust restructure of images when the pixels are overlapped. Micro-biochip with protein binding and DNA amplification could be detected by using this spectral scanning super-resolution molecules imaging in label free. This method has several advantages as following: Firstly, the decoding and detecting steps are combined into one step. It makes tests faster and easier. Secondly, we used thickness-coded, minimized chips instead of a large microarray chip to carry the probes. This accelerates the interaction of the biomolecules. Thirdly, since only one kind of probes are attached to our thickness-coded, minimized chip, users can only pick out the probes they are interested in for a test without wasting unnecessary probes and chips.

Paper Details

Date Published: 12 March 2014
PDF: 4 pages
Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 894917 (12 March 2014); doi: 10.1117/12.2038462
Show Author Affiliations
Junqi Zhang, Tsinghua Univ. School of Medicine (China)
Qi Li, Tsinghua Univ. School of Medicine (China)
Rongxin Fu, Tsinghua Univ. School of Medicine (China)
Tongzhou Wang, Tsinghua Univ. School of Medicine (China)
Ruliang Wang, Tsinghua Univ. School of Medicine (China)
Guoliang Huang, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)


Published in SPIE Proceedings Vol. 8949:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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