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Proceedings Paper

Multiphoton imaging of biological samples during freezing and heating
Author(s): H. G. Breunig; A. Uchugonova; K. König
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Paper Abstract

We applied multiphoton microscopic imaging to observe freezing and heating effects in plant- and animal cell samples. The experimental setups consisted of a multiphoton imaging system and a heating and cooling stage which allows for precise temperature control from liquid nitrogen temperature (-196°C; 77 K) up to +600°C (873 K) with heating/freezing rates between 0.01 K/min and 150 K/min. Two multiphoton imaging systems were used: a system based on a modified optical microscope and a flexible mobile system. To illustrate the imaging capabilities, plant leafs as well as animal cells were microscopically imaged in vivo during freezing based on autofluorescence lifetime and intensity of intrinsic molecules. The measurements illustrate the usefulness of multiphoton imaging to investigate freezing effects on animal and plant cells.

Paper Details

Date Published: 28 February 2014
PDF: 7 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894819 (28 February 2014); doi: 10.1117/12.2038452
Show Author Affiliations
H. G. Breunig, JenLab GmbH (Germany)
Saarland Univ. (Germany)
A. Uchugonova, JenLab GmbH (Germany)
K. König, JenLab GmbH (Germany)
Saarland Univ. (Germany)

Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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