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Proceedings Paper

Recombinant Adeno-associated virus (rAAV)-mediated transduction and optogenetic manipulation of cortical neurons in vitro
Author(s): Wienke Lange; Lei Jin; Vanessa Maybeck; Annika Meisenberg; Arnd Baumann; Andreas Offenhäusser
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Paper Abstract

Genetically encoded light-sensitive proteins can be used to manipulate and observe cellular functions. According to different modes of action, these proteins are divided into actuators like the blue-light gated cation channel Channelrhodopsin-2 (ChR2) and detectors like the calcium sensor GCaMP. In order to optogenetically control and study the activity of rat primary cortical neurons, we established a transduction procedure using recombinant Adeno-associated viruses (rAAVs) as gene-ferries. Thereby, we achieved high transduction rates of these neurons with ChR2. In ChR2 expressing neurons, action potentials could be repeatedly and precisely elicited with laser pulses and measured via patch clamp recording.

Paper Details

Date Published: 24 March 2014
PDF: 8 pages
Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89282S (24 March 2014); doi: 10.1117/12.2038437
Show Author Affiliations
Wienke Lange, Forschungszentrum Jülich GmbH (Germany)
Lei Jin, Forschungszentrum Jülich GmbH (Germany)
Vanessa Maybeck, Forschungszentrum Jülich GmbH (Germany)
Annika Meisenberg, Forschungszentrum Jülich GmbH (Germany)
Arnd Baumann, Forschungszentrum Jülich GmbH (Germany)
Andreas Offenhäusser, Forschungszentrum Jülich GmbH (Germany)

Published in SPIE Proceedings Vol. 8928:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
Henry Hirschberg; Steen J. Madsen; E. Duco Jansen; Qingming Luo; Samarendra K. Mohanty; Nitish V. Thakor, Editor(s)

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