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Proceedings Paper

Stepwise multi-photon activation fluorescence reveals a new method of melanoma imaging for dermatologists
Author(s): Zhenhua Lai; Christine Lian; Jie Ma; Jingyi Yu; Zetong Gu; Milind Rajadhyaksha; Charles A. DiMarzio
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Paper Abstract

Previous research has shown that the stepwise multi-photon activated fluorescence (SMPAF) of melanin, activated by a continuous-wave (CW) mode near infrared (NIR) laser, is a low cost and reliable method of detecting melanin. SMPAF images of melanin in a mouse hair and a formalin fixed mouse melanoma were compared with conventional multiphoton fluorescence microscopy (MPFM) images and confocal reflectance microscopy (CRM) images, all of which were acquired at an excitation wavelength of 920 nm, to further prove the effectiveness of SMPAF in detecting melanin. SMPAF images add specificity for melanin detection to MPFM images and CRM images. Melanin SMPAF can be a promising technology to enable melanoma imaging for dermatologists.

Paper Details

Date Published: 28 February 2014
PDF: 6 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482D (28 February 2014); doi: 10.1117/12.2038312
Show Author Affiliations
Zhenhua Lai, Northeastern Univ. (United States)
Bernard M. Gordon Ctr. for Subsurface Sensing and Imaging System (United States)
Christine Lian, Brigham and Women’s Hospital (United States)
Jie Ma, Brigham and Women’s Hospital (United States)
Jingyi Yu, Harvard Univ. (United States)
Zetong Gu, Northeastern Univ. (United States)
Bernard M. Gordon Ctr. for Subsurface Sensing and Imaging System (United States)
Milind Rajadhyaksha, Memorial and Sloan-Kettering Cancer Ctr. (United States)
Charles A. DiMarzio, Northeastern Univ. (United States)
Bernard M. Gordon Ctr. for Subsurface Sensing and Imaging System (United States)


Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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