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Proceedings Paper

Fluorescent cyanine probe for DNA detection and cellular imaging
Author(s): Yong-Chao Zheng; Mei-Ling Zheng; Zhen-Sheng Zhao; Xuan-Ming Duan
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Paper Abstract

In our study, two carbazole-based cyanines, 3,6-bis[2-(1-methylpyridinium)vinyl]-9-methyl carbazole diiodide (A) and 6,6'-bis[2-(1-methylpyridinium)vinyl]-bis(9-methyl-carbazol-3yl)methane diiodide (B) were synthesized and employed as light-up probes for DNA and cell imaging. Both of the cyanine probes possess a symmetric structure and bis-cationic center. The obvious induced circular dichroism signals in circular dichroism spectra reveal that the molecules can specifically interact with DNA. Strong fluorescence enhancement is observed when these two cyanines are bound to DNA. These cyanine probes show high binding affinity to oligonucleotides but different binding preferences to various secondary structures. Confocal microscopy images of fixed cell stained by the probes exhibit strong brightness and high contrast in nucleus with a very low cytoplasmic background.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 894722 (4 March 2014); doi: 10.1117/12.2038274
Show Author Affiliations
Yong-Chao Zheng, Technical Institute of Physics and Chemistry (China)
Univ. of Chinese Academy of Sciences (China)
Mei-Ling Zheng, Technical Institute of Physics and Chemistry (China)
Zhen-Sheng Zhao, Technical Institute of Physics and Chemistry (China)
Xuan-Ming Duan, Technical Institute of Physics and Chemistry (China)
Chongqing Institute of Green and Intelligent Technology (China)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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