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Proceedings Paper

Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice
Author(s): Jaepyeong Cha; Martin Paukert; Dwight E. Bergles; Jin U. Kang
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Paper Abstract

Fiber-optic based optical imaging is an emerging technique for studying brain activity in live animals. Here, we introduce a novel fluorescence fiber-optic microendoscopy approach to minimal invasively detect neural activities in a live mouse brain . The system uses a flexible endoscopic probe composed of a multi-core coherent fiber-bundle terminated with an approximately 1500-micron working distance objective lens. The fiber-optic neural interface is mounted on a 4-mm2 cranial window enabling visualization of glial calcium transients from the same brain region for weeks. We evaluated the system performance through in vivo imaging of GCaMP3 fluorescence in transgenic headrestrained mice during locomotion.

Paper Details

Date Published: 20 March 2014
PDF: 6 pages
Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89282N (20 March 2014); doi: 10.1117/12.2038265
Show Author Affiliations
Jaepyeong Cha, Johns Hopkins Univ. (United States)
Martin Paukert, Johns Hopkins School of Medicine (United States)
The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Dwight E. Bergles, Johns Hopkins School of Medicine (United States)
Jin U. Kang, Johns Hopkins Univ. (United States)

Published in SPIE Proceedings Vol. 8928:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
Henry Hirschberg; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Steen J. Madsen, Editor(s)

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