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Proceedings Paper

Photothermal therapy of human glioma spheroids with gold-silica nanoshells and gold nanorods: a comparative study
Author(s): Suyog Chhetri; Henry Hirschberg; Steen J. Madsen
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Paper Abstract

The efficacy of gold-silica nanoshells (AuNS) and gold nanorods (AuNR) for photothermal therapy was investigated in an in vitro system consisting of hybrid murine macrophage/human glioma spheroids. Macrophages were used as delivery vectors for the nanoparticles. Hybrid spheroids were formed via centrifugation of human glioma cells and nanoparticleloaded macrophages. Forty-eight hours post-centrifugation, the resultant 400 μm dia. spheroids were exposed to 808 nm laser light for 10 min. at irradiances ranging from 2 - 28 W cm-2. Treatment efficacy was evaluated from spheroid growth kinetics over a 14-day period. AuNS were shown to have greater efficacy compared to AuNR. For example, hybrid spheroids consisting of a 5:1 ratio of glioma cells to AuNS-loaded macrophages exhibited significant growth inhibition when subjected to irradiances of 7 W cm-2. In contrast, no growth inhibition was observed for the AuNR-macrophage hybrid spheroids, even at the highest irradiance investigated (28 W cm-2). Growth inhibition was observed at 28 W cm-2 when the nanorod concentration was increased, i.e., by forming hybrid spheroids with a 2:1 ratio of glioma cells to macrophages.

Paper Details

Date Published: 5 March 2014
PDF: 8 pages
Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89280U (5 March 2014); doi: 10.1117/12.2038233
Show Author Affiliations
Suyog Chhetri, Univ. of Nevada, Las Vegas (United States)
Henry Hirschberg, Beckman Laser Institute and Medical Clinic (United States)
Steen J. Madsen, Univ. of Nevada, Las Vegas (United States)

Published in SPIE Proceedings Vol. 8928:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
Henry Hirschberg; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Steen J. Madsen, Editor(s)

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