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Proceedings Paper

Video lensfree microscopy of 2D and 3D culture of cells
Author(s): C. P. Allier; S. Vinjimore Kesavan; J.-G. Coutard; O. Cioni; F. Momey; F. Navarro; M. Menneteau; B. Chalmond; P. Obeid; V. Haguet; B. David-Watine; N. Dubrulle; S. Shorte; B. van der Sanden; C. Di Natale; L. Hamard; D. Wion; M. E. Dolega; N. Picollet-D'hahan; X. Gidrol; J.-M. Dinten
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Paper Abstract

Innovative imaging methods are continuously developed to investigate the function of biological systems at the microscopic scale. As an alternative to advanced cell microscopy techniques, we are developing lensfree video microscopy that opens new ranges of capabilities, in particular at the mesoscopic level. Lensfree video microscopy allows the observation of a cell culture in an incubator over a very large field of view (24 mm2) for extended periods of time. As a result, a large set of comprehensive data can be gathered with strong statistics, both in space and time. Video lensfree microscopy can capture images of cells cultured in various physical environments. We emphasize on two different case studies: the quantitative analysis of the spontaneous network formation of HUVEC endothelial cells, and by coupling lensfree microscopy with 3D cell culture in the study of epithelial tissue morphogenesis. In summary, we demonstrate that lensfree video microscopy is a powerful tool to conduct cell assays in 2D and 3D culture experiments. The applications are in the realms of fundamental biology, tissue regeneration, drug development and toxicology studies.

Paper Details

Date Published: 4 March 2014
PDF: 9 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471H (4 March 2014); doi: 10.1117/12.2038098
Show Author Affiliations
C. P. Allier, CEA-LETI (France)
S. Vinjimore Kesavan, CEA-LETI (France)
J.-G. Coutard, CEA-LETI (France)
O. Cioni, CEA-LETI (France)
F. Momey, CEA-LETI (France)
F. Navarro, CEA-LETI (France)
M. Menneteau, CEA-LETI (France)
B. Chalmond, Univ. de Cergy-Pontoise and CMLA (France)
Ecole Normale Supérieure de Cachan (France)
P. Obeid, Lab. BTE, Institut de Recherches en Technologies et Sciences (France)
INSERM (France)
Univ. Grenoble-Alpes (France)
V. Haguet, Lab. BTE, Institut de Recherches en Technologies et Sciences (France)
INSERM (France)
Univ. Grenoble-Alpes (France)
B. David-Watine, Institut Pasteur (France)
N. Dubrulle, Institut Pasteur (France)
S. Shorte, Institut Pasteur (France)
B. van der Sanden, CEA Clinatec (France)
C. Di Natale, CEA Clinatec (France)
L. Hamard, CEA Clinatec (France)
D. Wion, CEA Clinatec (France)
M. E. Dolega, Lab. BTE, Institut de Recherches en Technologies et Sciences (France)
INSERM (France)
Univ. Grenoble-Alpes (France)
N. Picollet-D'hahan, Lab. BTE, Institut de Recherches en Technologies et Sciences (France)
INSERM (France)
Univ. Grenoble-Alpes (France)
X. Gidrol, Lab. BTE, Institut de Recherches en Technologies et Sciences (France)
INSERM (France)
Univ. Grenoble-Alpes (France)
J.-M. Dinten, CEA-LETI (France)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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