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Proceedings Paper

Micovascular integration into porous polyHEMA scaffold
Author(s): Eugenia H. Cho; Alina Boico; Natalie A. Wisniewski; Rebecca Gant; Kristen L. Helton; Nga L. Brown; Janna K. Register; Tuan Vo-Dinh; Thies Schroeder; Bruce Klitzman
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Paper Abstract

Surface-enhanced Raman scattering (SERS) spectroscopy can be a useful tool in regard to disease diagnosis and prevention. Advantage of SERS over conventional Raman spectroscopy is its significantly increased signal (up to factor of 106-108) which allows detection of trace amounts of substances in the sample. So far, this technique is successfully used for analysis of food, pieces of art and various biochemical/biomedical samples. In this work, we survey the possibility of applying SERS spectroscopy for detection of trace components in urinary deposits. Early discovery together with the identification of the exact chemical composition of urinary sediments could be crucial for taking appropriate preventive measures that inhibit kidney stone formation or growth processes. In this initial study, SERS spectra (excitation wavelength - 1064 nm) of main components of urinary deposits (calcium oxalate, uric acid, cystine, etc.) were recorded by using silver (Ag) colloid. Spectra of 10-3-10-5 M solutions were obtained. While no/small Raman signal was detected without the Ag colloid, characteristic peaks of the substances could be clearly separated in the SERS spectra. This suggests that even small amounts of the components could be detected and taken into account while determining the type of kidney stone forming in the urinary system. We found for the first time that trace amounts of components constituting urinary deposits could be detected by SERS spectroscopy. In the future study, the analysis of centrifuged urine samples will be carried out.

Paper Details

Date Published: 3 March 2014
PDF: 4 pages
Proc. SPIE 8958, Bioinspired, Biointegrated, Bioengineered Photonic Devices II, 89580B (3 March 2014); doi: 10.1117/12.2037950
Show Author Affiliations
Eugenia H. Cho, Duke Univ. (United States)
Alina Boico, Duke Univ. (United States)
Natalie A. Wisniewski, PROFUSA, Inc. (United States)
Rebecca Gant, PROFUSA, Inc. (United States)
Kristen L. Helton, PROFUSA, Inc. (United States)
Nga L. Brown, Duke Univ. (United States)
Janna K. Register, Duke Univ. (United States)
Tuan Vo-Dinh, Duke Univ. (United States)
Thies Schroeder, Duke Univ. (United States)
Bruce Klitzman, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 8958:
Bioinspired, Biointegrated, Bioengineered Photonic Devices II
Luke P. Lee; John A. Rogers; Seok Hyun Andy Yun, Editor(s)

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