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Proceedings Paper

Optical characterization of ex-vivo axillary lymph nodes of breast-cancer patients using a custom-built spectrophotometer
Author(s): Ashwin Sampathkumar; Emi Saegusa-Beecroft; Jonathan Mamou; Parag V. Chitnis; Junji Machi; Ernest J. Feleppa
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Paper Abstract

Quantitative photoacoustics is emerging as a new hybrid modality to investigate diseases and cells in human pathology and cytology studies. Optical absorption of light is the predominant mechanism behind the photoacoustic effect. Therefore, a need exits to characterize the optical properties of specimens and to identify the relevant operating wavelengths for photoacoustic imaging. We have developed a custom low-cost spectrophotometer to measure the optical properties of human axillary lymph nodes dissected for breast-cancer staging. Optical extinction curves of positive and negative nodes were determined in the spectral range of 400 to 1000 nm. We have developed a model to estimate tissue optical properties, taking into account the role of fat and saline. Our results enabled us to select the optimal optical wavelengths for maximizing the imaging contrast between metastatic and noncancerous tissue in axillary lymph nodes.

Paper Details

Date Published: 17 March 2014
PDF: 6 pages
Proc. SPIE 8940, Optical Biopsy XII, 89400Q (17 March 2014); doi: 10.1117/12.2037864
Show Author Affiliations
Ashwin Sampathkumar, F.L. Lizzi Ctr. for Biomedical Engineering, Riverside Research Institute (United States)
Emi Saegusa-Beecroft, Univ. of Hawai'i (United States)
Kuakani Medical Ctr. (United States)
Jonathan Mamou, F.L. Lizzi Ctr. for Biomedical Engineering, Riverside Research Institute (United States)
Parag V. Chitnis, F.L. Lizzi Ctr. for Biomedical Engineering, Riverside Research Institute (United States)
Junji Machi, Univ. of Hawai'i (United States)
Kuakani Medical Ctr. (United States)
Ernest J. Feleppa, F.L. Lizzi Ctr. for Biomedical Engineering, Riverside Research Institute (United States)


Published in SPIE Proceedings Vol. 8940:
Optical Biopsy XII
Robert R. Alfano; Stavros G. Demos, Editor(s)

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