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Proceedings Paper

Investigation on laser induced salivary stone fragmentation
Author(s): Ronald Sroka; Thomas Pongratz; Matthias Eder; Mona Domes; Michael Vogeser; Thorsten Johnson; Vanessa Siedeck; Florian Schroetzlmair; Pamela Zengel
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Paper Abstract

Objective: It was the objective of this in-vitro study to investigate photon-based techniques for identifying the composition and fragmentation of salivary stones using a Ho:YAG laser. Materials and Method: Salivary stones (n=47) extracted from patients with clinical symptoms of sialolithiasis were examined in-vitro. After extraction, the stones were kept in Ringers solution until size and volume measurements could be performed. Thereafter, dual-energy CT scans (DECT) were performed to classify the composition of the stones. Subsequently, fluorescence measurements were performed by taking images under blue light excitation as well as by fluorescence spectroscopy, measuring excitation-emission-matrixes (EEM). Further investigation to identify the exact composition of the stone was performed by Raman spectroscopy and FTIR spectroscopy of stone fragments and debris. Fragmentation was performed in an aquarium set-up equipped with a mesh (hole: 1.5mm) using a Ho:YAG-laser to deliver laser pulses of 0.5, 1.0 and 1.5J/pulse at a frequency of 3Hz through a 200μm-fibre to the stone surface. The collected data were analyzed and fragmentation rates were calculated. Finally, correlation between stone composition and fragmentation was performed. Results: Blue light fluorescence excitation resulted in either fluorescence in the green spectral region or in a combination of green and red fluorescence emission. EEM-measurement showed the corresponding spectra. Raman spectroscopy showed a mixture of carbonate apatite and keratin. DECT results in evidence of calcium containing components. FTIR-spectroscopy results showed that carbonate apatite is the main component. Fragmentation experiment showed a dependency on the energy per pulse applied if the evaluation implies the ratio of fragmented weight to pulse, while the ratio fragmented weight to energy remains about constant for the three laser parameter used. Conclusion: The composition of salivary stones could be determined using different photonic techniques. Attempts to correlate salivary stone composition to fragmentation rates resulted in no correlation. Thus it could be concluded that each salivary stone could be easily destroyed using Ho:YAG-laser light by means of a 200μm bare fibre at lowest energy per pulse.

Paper Details

Date Published: 4 March 2014
PDF: 5 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89262E (4 March 2014); doi: 10.1117/12.2037850
Show Author Affiliations
Ronald Sroka, Klinikum der Univ. München (Germany)
Thomas Pongratz, Klinikum der Univ. München (Germany)
Matthias Eder, Klinikum der Univ. München (Germany)
Mona Domes, Klinikum der Univ. München (Germany)
Michael Vogeser, Klinikum der Univ. München (Germany)
Thorsten Johnson, Klinikum der Univ. München (Germany)
Vanessa Siedeck, Klinikum der Univ. München (Germany)
Florian Schroetzlmair, Klinikum der Univ. München (Germany)
Pamela Zengel, Klinikum der Univ. München (Germany)


Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong; Guillermo J. Tearney; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory; Justus F. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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