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Proceedings Paper

The study of hydrogen peroxide level under cisplatin action using genetically encoded sensor hyper
Author(s): A. S. Belova; A. G. Orlova; A. V. Maslennikova; A. A. Brilkina; I. V. Balalaeva; N. O. Antonova; N. M. Mishina; N. M. Shakhova; V. V. Belousov
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Paper Abstract

The aim of the work was to study the participation of hydrogen peroxide in reaction of cervical cancer cell line HeLa Kyoto on cisplatin action. Determination of hydrogen peroxide level was performed using genetically encoded fluorescent sensor HyPer2. The dependence of cell viability on cisplatin concentration was determined using MTT assay. Mechanisms of cell death as well as HyPer2 reaction was revealed by flow cytometry after 6-hours of incubation with cisplatin in different concentrations. Cisplatin used in low concentrations had no effect on hydrogen peroxide level in HeLa Kyoto cells. Increase of HyPer2 fluorescence was detected only after exposure with cisplatin in high concentration. The reaction was not the consequence of cell death.

Paper Details

Date Published: 7 March 2014
PDF: 6 pages
Proc. SPIE 8956, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VI, 895612 (7 March 2014); doi: 10.1117/12.2037737
Show Author Affiliations
A. S. Belova, N.I. Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation)
A. G. Orlova, N.I. Lobachevsky State University of Nizhny Novgorod (Russian Federation)
Institute of Applied Physics (Russian Federation)
A. V. Maslennikova, N.I. Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation)
Institute of Applied Physics (Russian Federation)
Nizhny Novgorod State Medical Academy (Russian Federation)
A. A. Brilkina, N.I. Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation)
I. V. Balalaeva, N.I. Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation)
N. O. Antonova, N.I. Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation)
N. M. Mishina, Nizhny Novgorod State Medical Academy (Russian Federation)
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
N. M. Shakhova, N.I. Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation)
Institute of Applied Physics (Russian Federation)
Nizhny Novgorod State Medical Academy (Russian Federation)
V. V. Belousov, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)


Published in SPIE Proceedings Vol. 8956:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VI
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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