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Proceedings Paper

Ptychography: use of quantitative phase information for high-contrast label free time-lapse imaging of living cells
Author(s): Rakesh Suman; Peter O'Toole
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Paper Abstract

Here we report a novel label free, high contrast and quantitative method for imaging live cells. The technique reconstructs an image from overlapping diffraction patterns using a ptychographical algorithm. The algorithm utilises both amplitude and phase data from the sample to report on quantitative changes related to the refractive index (RI) and thickness of the specimen. We report the ability of this technique to generate high contrast images, to visualise neurite elongation in neuronal cells, and to provide measure of cell proliferation.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471Z (4 March 2014); doi: 10.1117/12.2037670
Show Author Affiliations
Rakesh Suman, Univ. of York (United Kingdom)
Peter O'Toole, Univ. of York (United Kingdom)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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