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Proceedings Paper

Fluorescence lifetime imaging of induced pluripotent stem cells
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Paper Abstract

The multiphoton FLIM tomograph MPTflex with its flexible scan head, articulated arm, and the tunable femtosecond laser source was employed to study cell monolayers and 3D cell clusters. FLIM was performed with 250 ps temporal resolution and submicron special resolution using time-correlated single photon counting. The autofluorescence based on NAD(P)H and flavins/flavoproteins has been measured in mouse embryonic fibroblasts, induced pluripotent stem cells (iPS cells) originated from mouse embryonic fibroblasts and non-proliferative mouse embryonic fibroblasts.

Paper Details

Date Published: 28 February 2014
PDF: 7 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89481I (28 February 2014); doi: 10.1117/12.2037662
Show Author Affiliations
Aisada Uchugonova, Saarland Univ. (Germany)
JenLab GmbH (Germany)
Ana Batista, Saarland Univ. (Germany)
Karsten König, Saarland Univ. (Germany)
JenLab GmbH (Germany)


Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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