Share Email Print
cover

Proceedings Paper

Cell identification using Raman spectroscopy in combination with optical trapping and microfluidics
Author(s): Christoph Krafft; Sebastian Dochow; Claudia Beleites; Jürgen Popp
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Cell identification by Raman spectroscopy has evolved to be an attractive complement to established optical techniques. Raman activated cell sorting (RACS) offers prospects to complement the widely applied fluorescence activated cell sorting. RACS can be realized by combination with optical traps and microfluidic devices. The progress of RACS is reported for a cellular model system that can be found in peripheral blood of tumor patients. Lymphocytes and erythrocytes were extracted from blood samples. Breast carcinoma derived tumor cells (MCF-7, BT-20) and acute myeloid leukemia cells (OCI-AML3) were grown in cell cultures. First, Raman images were collected from dried cells on calcium fluoride slides. Support vector machines (SVM) classified 99.7% of the spectra to the correct cell type. Second, a 785 nm laser was used for optical trapping of single cells in aqueous buffer and for excitation of the Raman spectrum. SVM distinguished 1210 spectra of tumor and normal cells with a sensitivity of >99.7% and a specificity of >99.5%. Third, a microfluidic glass chip was designed to inject single cells, modify the flow speed, accommodate fibers of an optical trap and sort single cells after Raman based identification with 514 nm for excitation. Forth, the microfluidic chip was fabricated by quartz which improved cell identification results with 785 nm excitation. Here, partial least squares discriminant analysis gave classification rates of 98%. Finally, a Raman-on-chip approach was developed that integrates fibers for trapping, Raman excitation and signal detection in a single compact unit.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 893906 (4 March 2014); doi: 10.1117/12.2037601
Show Author Affiliations
Christoph Krafft, Institut für Photonische Technologien e.V. (Germany)
Sebastian Dochow, Institut für Photonische Technologien e.V. (Germany)
Claudia Beleites, Institut für Photonische Technologien e.V. (Germany)
Jürgen Popp, Institut für Photonische Technologien e.V. (Germany)
Univ. Jena (Germany)


Published in SPIE Proceedings Vol. 8939:
Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

© SPIE. Terms of Use
Back to Top