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Proceedings Paper

Correction of image artifacts caused by refractive index gradients in scanning laser optical tomography
Author(s): Georgios Christian Antonopoulos; Dimitri Pscheniza; Raoul-Amadeus Lorbeer; Marko Heidrich; Kristin Schwanke; Robert Zweigerdt; Tammo Ripken; Heiko Meyer
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Paper Abstract

We present a technique for correcting image artifacts caused by refractive index distributions in Scanning Laser Optical Tomography (SLOT) and Optical Projection Tomography (OPT). Projection images can be distorted due to the presence of a refractive index distribution around the sample. We consider the special case of a refractive index distribution given by a capillary around a sample. The particular application we are interested in is in vitro imaging of cell spheroids in a glass capillary. Numerical simulations and experimental results are used to illustrate the connection between the Radon transform and the refracted projection. Thereupon we will describe a technique that transforms refracted projections to parallel ray Radon projections and thus allows artifact free reconstruction within the sample volume.

Paper Details

Date Published: 12 March 2014
PDF: 6 pages
Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 894907 (12 March 2014); doi: 10.1117/12.2037581
Show Author Affiliations
Georgios Christian Antonopoulos, Laser Zentrum Hannover e.V. (Germany)
Dimitri Pscheniza, Leibniz Research Labs. for Biotechnology and Artificial Organs, Hannover Medical School (Germany)
Raoul-Amadeus Lorbeer, Laser Zentrum Hannover e.V. (Germany)
Marko Heidrich, Laser Zentrum Hannover e.V. (Germany)
Kristin Schwanke, Leibniz Research Labs. for Biotechnology and Artificial Organs, Hannover Medical School (Germany)
Robert Zweigerdt, Leibniz Research Labs. for Biotechnology and Artificial Organs, Hannover Medical School (Germany)
Tammo Ripken, Laser Zentrum Hannover e.V. (Germany)
Heiko Meyer, Laser Zentrum Hannover e.V. (Germany)
HTTG, Hannover Medical School (Germany)


Published in SPIE Proceedings Vol. 8949:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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