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Proceedings Paper

Holographic approach for monitoring of the deactivation of excited biomolecules
Author(s): I. V. Semenova; O. S. Vasyutinskii; A. D. Moskovtseva; D. M. Maksimova
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Paper Abstract

The paper presents a novel approach to detect and monitor excited biomolecules by means of holographic registration of thermal disturbances produced by their radiationless deactivation. The photoacoustic and photorefractive methods do not provide any data on spatial distribution of these disturbances. Holographic interferometry allows one to obtain in a single shot a 2D image of the area under study containing information on spatial distribution of local variations of refractive index induced by temperature variations. The method feasibility is demonstrated on the monitoring of photosensitized generation and radiationless deactivation of singlet oxygen in water.

Paper Details

Date Published: 8 March 2014
PDF: 7 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89470N (8 March 2014); doi: 10.1117/12.2037305
Show Author Affiliations
I. V. Semenova, Ioffe Physico-Technical Institute (Russian Federation)
O. S. Vasyutinskii, Ioffe Physico-Technical Institute (Russian Federation)
St. Petersburg State Polytechnic Univ. (Russian Federation)
A. D. Moskovtseva, Ioffe Physico-Technical Institute (Russian Federation)
D. M. Maksimova, Ioffe Physico-Technical Institute (Russian Federation)
St. Petersburg State Polytechnic Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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