Share Email Print
cover

Proceedings Paper

Autofluorescence lifetime metrology for label-free detection of cartilage matrix degradation
Author(s): Mohammad B. Nickdel; João L. Lagarto; Douglas J. Kelly; Hugh B. Manning; Kazuhiro Yamamoto; Clifford B. Talbot; Christopher Dunsby; Paul French; Yoshifumi Itoh
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Degradation of articular cartilage extracellular matrix (ECM) by proteolytic enzyme is the hallmark of arthritis that leads to joint destruction. Detection of early biochemical changes in cartilage before irreversible structural damages become apparent is highly desirable. Here we report that the autofluorescence decay profile of cartilage is significantly affected by proteolytic degradation of cartilage ECM and can be characterised by measurements of the autofluorescence lifetime (AFL). A multidimensional fluorometer utilizing ultraviolet excitation at 355 nm or 375 nm coupled to a fibreoptic probe was developed for single point time-resolved AFL measurements of porcine articular cartilage explants treated with different proteinases. Degradation of cartilage matrix components by treating with bacterial collagenase, matrix metalloproteinase 1, or trypsin resulted in significant reduction of AFL of the cartilage in both a dose and time dependent manner. Differences in cartilage AFL were also confirmed by fluorescence lifetime imaging microscopy (FLIM). Our data suggest that AFL of cartilage tissue is a potential non-invasive readout to monitor cartilage matrix integrity that may be utilized for diagnosis of arthritis as well as monitoring the efficacy of anti-arthritic therapeutic agents.

Paper Details

Date Published: 17 March 2014
PDF: 8 pages
Proc. SPIE 8940, Optical Biopsy XII, 89400H (17 March 2014); doi: 10.1117/12.2037285
Show Author Affiliations
Mohammad B. Nickdel, Univ. of Oxford (United Kingdom)
João L. Lagarto, Imperial College London (United Kingdom)
Douglas J. Kelly, Imperial College London (United Kingdom)
Hugh B. Manning, Imperial College London (United Kingdom)
Kazuhiro Yamamoto, Univ. of Oxford (United Kingdom)
Clifford B. Talbot, Imperial College London (United Kingdom)
Christopher Dunsby, Imperial College London (United Kingdom)
Paul French, Imperial College London (United Kingdom)
Yoshifumi Itoh, Univ. of Oxford (United Kingdom)


Published in SPIE Proceedings Vol. 8940:
Optical Biopsy XII
Robert R. Alfano; Stavros G. Demos, Editor(s)

© SPIE. Terms of Use
Back to Top