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Proceedings Paper

Sensing vascularization of ex-vivo produced oral mucosal equivalent (EVPOME) skin grafts in nude mice using optical spectroscopy
Author(s): Karthik Vishwanath; Rajan Gurjar; Shiuhyang Kuo; Anthony Fasi; Roderick Kim; Suzannah Riccardi; Stephen E. Feinberg; David E. Wolf
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Paper Abstract

Repair of soft tissue defects of the lips as seen in complex maxillofacial injuries, requires pre-vascularized multi-tissue composite grafts. Protocols for fabrication of human ex-vivo produced oral mucosal equivalents (EVPOME) composed of epithelial cells and a dermal equivalent are available to create prelaminated flaps for grafting in patients. However, invivo assessment of neovascularization of the buried prelaminated flaps remains clinically challenging. Here, we use diffuse reflectance spectroscopy (DRS) and diffuse correlation spectroscopy (DCS) to non-invasively quantify longitudinal changes in the vessel density and blood-flow within EVPOME grafts implanted in the backs of SCID mice and subsequently to determine the utility of these optical techniques for assessing vascularization of implanted grafts. 20 animals were implanted with EVPOME grafts (1x1x0.05 cm3) in their backs. DRS and DCS measurements were obtained from each animal both atop the graft site and far away from the graft site, at one week post-implantation, each week, for four consecutive weeks. DRS spectra were analyzed using an inverse Monte Carlo model to extract tissue absorption and scattering coefficients, which were then used to extract blood flow information by fitting the experimental DCS traces. There were clear differences in the mean optical parameters (averaged across all mice) at the graft site vs. the off-site measurements. Both the total hemoglobin concentration (from DRS) and the relative blood flow (from DCS) peaked at week 3 at the graft site and declined to the off-site values by week 4. The optical parameters remained relatively constant throughout 4 weeks for the off-site measurements.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89260I (4 March 2014); doi: 10.1117/12.2037085
Show Author Affiliations
Karthik Vishwanath, Radiation Monitoring Devices, Inc. (United States)
Rajan Gurjar, Radiation Monitoring Devices, Inc. (United States)
Shiuhyang Kuo, Univ. of Michigan (United States)
Anthony Fasi, Univ. of Michigan (United States)
Roderick Kim, Univ. of Michigan (United States)
Suzannah Riccardi, Radiation Monitoring Devices, Inc. (United States)
Stephen E. Feinberg, Univ. of Michigan (United States)
David E. Wolf, Radiation Monitoring Devices, Inc. (United States)


Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong; Guillermo J. Tearney; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory; Justus F. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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