Share Email Print
cover

Proceedings Paper

Miniaturized CARS microendoscope probe design for label-free intraoperative imaging
Author(s): Xu Chen; Xi Wang; Xiaoyun Xu; Jie Cheng; Zhengfan Liu; Sheng Weng; Michael J. Thrall; Alvin C. Goh; Daniel T. McCormick; Kelvin Wong; Stephen T. C. Wong
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

A Coherent Anti-Stokes Raman Scattering (CARS) microendoscope probe for early stage label-free prostate cancer diagnosis at single cell resolution is presented. The handheld CARS microendoscope probe includes a customized micro-electromechanical systems (MEMS) scanning mirror as well as miniature optical and mechanical components. In our design, the excitation laser (pump and stokes beams) from the fiber is collimated, reflected by the reflecting mirror, and transmitted via a 2D MEMS scanning mirror and a micro-objective system onto the sample; emission in the epi-direction is returned through the micro-objective lens, MEMS and reflecting mirror, and collimation system, and finally the emission signal is collected by a photomultiplier tube (PMT). The exit pupil diameter of the collimator system is designed to match the diameter of the MEMS mirror and the entrance pupil diameter of the micro-objective system. The back aperture diameter of the micro-objective system is designed according to the largest MEMS scanning angle and the distance between the MEMS mirror and the back aperture. To increase the numerical aperture (NA) of the micro-objective system in order to enhance the signal collection efficiency, the back aperture diameter of the micro-objective system is enlarged with an upfront achromatic wide angle Keplerian telescope beam expander. The integration of a miniaturized micro-optics probe with optical fiber CARS microscopy opens up the possibility of in vivo molecular imaging for cancer diagnosis and surgical intervention.

Paper Details

Date Published: 4 March 2014
PDF: 9 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89470Y (4 March 2014); doi: 10.1117/12.2037063
Show Author Affiliations
Xu Chen, The Methodist Hospital Research Institute (United States)
Xi Wang, The Methodist Hospital Research Institute (United States)
Xiaoyun Xu, The Methodist Hospital Research Institute (United States)
Jie Cheng, The Methodist Hospital Research Institute (United States)
Zhengfan Liu, The Methodist Hospital Research Institute (United States)
Sheng Weng, The Methodist Hospital Research Institute (United States)
Rice Univ. (United States)
Michael J. Thrall, Houston Methodist Hospital (United States)
Alvin C. Goh, Houston Methodist Hospital (United States)
Daniel T. McCormick, Advanced MEMS (United States)
Kelvin Wong, The Methodist Hospital Research Institute (United States)
Houston Methodist Hospital (United States)
Stephen T. C. Wong, The Methodist Hospital Research Institute (United States)
Houston Methodist Hospital (United States)
Rice Univ. (United States)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top