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Proceedings Paper

Multimodal fiber probe spectroscopy for tissue diagnostics applications: a combined Raman-fluorescence approach
Author(s): Riccardo Cicchi; Suresh Anand; Susanna Rossari; Alessandro Sturiale; Flavio Giordano; Vincenzo De Giorgi; Vincenza Maio; Daniela Massi; Gabriella Nesi; Anna Maria Buccoliero; Francesco Tonelli; Renzo Guerrini; Nicola Pimpinelli; Francesco Saverio Pavone
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Paper Abstract

Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnosing melanocytic lesions in a good agreement with common routine histology. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. Further investigations were performed on colon and brain tissue samples in order to have a benchmark for diagnosing a broader range of tissue lesions and malignancies. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390U (4 March 2014); doi: 10.1117/12.2037024
Show Author Affiliations
Riccardo Cicchi, Istituto Nazionale di Ottica-CNR (Italy)
European Lab. for Non-linear Spectroscopy (Italy)
Suresh Anand, European Lab. for Non-linear Spectroscopy (Italy)
Susanna Rossari, Univ. degli Studi di Firenze (Italy)
Alessandro Sturiale, Univ. degli Studi di Firenze (Italy)
Flavio Giordano, “Anna Meyer” Pediatric Hospital (Italy)
Vincenzo De Giorgi, Univ. degli Studi di Firenze (Italy)
Vincenza Maio, Univ. degli Studi di Firenze (Italy)
Daniela Massi, Univ. degli Studi di Firenze (Italy)
Gabriella Nesi, Univ. degli Studi di Firenze (Italy)
Anna Maria Buccoliero, Univ. degli Studi di Firenze (Italy)
Francesco Tonelli, Univ. degli Studi di Firenze (Italy)
Renzo Guerrini, “Anna Meyer” Pediatric Hospital (Italy)
Nicola Pimpinelli, Univ. degli Studi di Firenze (Italy)
Francesco Saverio Pavone, European Lab. for Non-linear Spectroscopy (Italy)
Univ. degli Studi di Firenze (Italy)


Published in SPIE Proceedings Vol. 8939:
Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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