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Proceedings Paper

Real-time optoacoustic monitoring of stroke
Author(s): Moritz Kneipp; Jake Turner; Sebastian Hambauer; Sandro M. Krieg; Jens Lehmberg; Ute Lindauer; Daniel Razansky
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Paper Abstract

Characterizing disease progression and identifying possible therapeutic interventions in stroke is greatly aided by the use of longitudinal function imaging studies. In this study, we investigate the applicability of real-time multispectral optoacoustic tomography (MSOT) as a tool for non-invasive monitoring of the progression of stroke in the whole brain. The middle cerebral artery occlusion (MCAO) method was used to induce stroke. Mice were imaged under isoflurane anesthesia preoperatively and at several time points during and after the 60-minute occlusion. The animals were sacrificed after 24 hours and their excised brains frozen at -80°C for sectioning. The cryosection were stained using H&E staining to identify the ischemic lesion. Major vessels are readily identifiable in the whole mouse head in the in vivo optoacoustic scans. During ischemia, a reduction in cerebral blood volume is detectable in the cortex. Post ischemia, spectral unmixing of the optoacoustic signals shows an asymmetry of the deoxygenated hemoglobin in the hemisphere affected by MCAO. This hypoxic area was mainly located around the boundary of the ischemic lesion and was therefore identified as the ischemic penumbra. Non-invasive functional MSOT imaging is able to visualize the hypoxic penumbra in brains affected by stroke. Stopping the spread of the infarct area and revitalizing the penumbra is central in stroke research, this new imaging technique may therefore prove to be a valuable tool in the monitoring and developing new treatments.

Paper Details

Date Published: 3 March 2014
PDF: 5 pages
Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89430L (3 March 2014); doi: 10.1117/12.2036948
Show Author Affiliations
Moritz Kneipp, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Jake Turner, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Sebastian Hambauer, Technische Univ. München (Germany)
Sandro M. Krieg, Technische Univ. München (Germany)
Jens Lehmberg, Technische Univ. München (Germany)
Ute Lindauer, Technische Univ. München (Germany)
SyNergy (Germany)
Daniel Razansky, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)


Published in SPIE Proceedings Vol. 8943:
Photons Plus Ultrasound: Imaging and Sensing 2014
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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