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Proceedings Paper

In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy
Author(s): Nirmal Mazumder; Lu Yun Xiang; Jianjun Qiu; Fu-Jen Kao
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Paper Abstract

We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.

Paper Details

Date Published: 28 February 2014
PDF: 14 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894822 (28 February 2014); doi: 10.1117/12.2036651
Show Author Affiliations
Nirmal Mazumder, National Yang-Ming Univ. (Taiwan)
Lu Yun Xiang, National Yang-Ming Univ. (Taiwan)
Jianjun Qiu, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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