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Proceedings Paper

Interpreting CARS images of tissue within the C-H-stretching region
Author(s): Benjamin Dietzek; Tobias Meyer; Anna Medyukhina; Norbert Bergner; Christoph Krafft; Bernd F. M. Romeike; Rupert Reichart; Rolf Kalff; Michael Schmitt; Jürgen Popp
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Paper Abstract

Single band coherent anti-Stokes Raman scattering (CARS) microscopy within the CH-stretching region is applied to detect individual cells and nuclei of human brain tissue and brain tumors – an information which allows for histopathologic grading of the tissue. The CARS image contrast within the C-H-stretching region correlated to the tissue composition. Based on the specific application example of identifying nuclei within (coherent) Raman images of neurotissue sections, we shall derive general design parameters for lasers optimally suited to serve in a clinical environment and discuss the potential of recently developed methods to analyze spectrally resolved CARS images and image segmentation algorithms.

Paper Details

Date Published: 20 March 2014
PDF: 7 pages
Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 892806 (20 March 2014); doi: 10.1117/12.2036547
Show Author Affiliations
Benjamin Dietzek, Institut für Photonische Technologien e.V. (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Tobias Meyer, Friedrich-Schiller-Univ. Jena (Germany)
Anna Medyukhina, Institut für Photonische Technologien e.V. (Germany)
Norbert Bergner, Institut für Photonische Technologien e.V. (Germany)
Christoph Krafft, Institut für Photonische Technologien e.V. (Germany)
Bernd F. M. Romeike, Friedrich-Schiller-Univ. Jena (Germany)
Rupert Reichart, Friedrich-Schiller-Univ. Jena (Germany)
Rolf Kalff, Friedrich-Schiller-Univ. Jena (Germany)
Michael Schmitt, Friedrich-Schiller-Univ. Jena (Germany)
Jürgen Popp, Institut für Photonische Technologien e.V. (Germany)
Friedrich-Schiller-Univ. Jena (Germany)


Published in SPIE Proceedings Vol. 8928:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
Henry Hirschberg; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Steen J. Madsen, Editor(s)

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