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Proceedings Paper

The application of surgical navigation system using optical molecular imaging technology in orthotopic breast cancer and metastasis studies
Author(s): Chongwei Chi; Qian Zhang; Deqiang Kou; Jinzuo Ye; Yamin Mao; Jingdan Qiu; Jiandong Wang; Xin Yang; Yang Du; Jie Tian
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Paper Abstract

Currently, it has been an international focus on intraoperative precise positioning and accurate resection of tumor and metastases. The methods such as X-rays, computed tomography (CT), magnetic resonance imaging (MRI) and positron emission tomography (PET) have played an important role in preoperative accurate diagnosis. However, most of them are inapplicable for intraoperative surgery. We have proposed a surgical navigation system based on optical molecular imaging technology for intraoperative detection of tumors and metastasis. This system collects images from two CCD cameras for real-time fluorescent and color imaging. For image processing, the template matching algorithm is used for multispectral image fusion. For the application of tumor detection, the mouse breast cancer cell line 4T1-luc, which shows highly metastasis, was used for tumor model establishment and a model of matrix metalloproteinase (MMP) expressing breast cancer. The tumor–bearing nude mice were given tail vein injection of MMP 750FAST (PerkinElmer, Inc. USA) probe and imaged with both bioluminescence and fluorescence to assess in vivo binding of the probe to the tumor and metastases sites. Hematoxylin and eosin (H&E) staining was performed to confirm the presence of tumor and metastasis. As a result, one tumor can be observed visually in vivo. However liver metastasis has been detected under surgical navigation system and all were confirmed by histology. This approach helps surgeons to find orthotopic tumors and metastasis during intraoperative resection and visualize tumor borders for precise positioning. Further investigation is needed for future application in clinics.

Paper Details

Date Published: 27 February 2014
PDF: 7 pages
Proc. SPIE 8935, Advanced Biomedical and Clinical Diagnostic Systems XII, 89350W (27 February 2014); doi: 10.1117/12.2036182
Show Author Affiliations
Chongwei Chi, Key Lab. of Molecular Imaging of Chinese Academy of Sciences (China)
Qian Zhang, Xidian Univ. (China)
Deqiang Kou, Chinese PLA General Hospital (China)
Jinzuo Ye, Key Lab. of Molecular Imaging of Chinese Academy of Sciences (China)
Yamin Mao, Key Lab. of Molecular Imaging of Chinese Academy of Sciences (China)
Jingdan Qiu, Chinese PLA General Hospital (China)
Jiandong Wang, Chinese PLA General Hospital (China)
Xin Yang, Key Lab. of Molecular Imaging of Chinese Academy of Sciences (China)
Yang Du, Key Lab. of Molecular Imaging of Chinese Academy of Sciences (China)
Jie Tian, Key Lab. of Molecular Imaging of Chinese Academy of Sciences (China)
Xidian Univ. (China)


Published in SPIE Proceedings Vol. 8935:
Advanced Biomedical and Clinical Diagnostic Systems XII
Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest, Editor(s)

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