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Proceedings Paper

Single-molecule fluorescence inside solid-state nanochannels
Author(s): Siddharth Ghosh; Manoj Kumbhakar; Mitja Platen; Ingo Gregor; Jörg Enderlein
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Paper Abstract

One major limitation of single-molecule fluorescence spectroscopy is the finite residence time of diffusing molecules in the confocal detection volume. The time ranges in the order of milliseconds. This has typical size of femtoliter. Here, we present a concept of extending the residence time using nanochannels of ca. 60 nm x 60 nm cross-section to restrict the molecular motion. We use solid-state nanochannels of silicon and silicon dioxide. This work is aimed to use for dual-focus fluorescence detection combining Anti-Brownian ELectrophoretic or ABEL trap to actively trap single molecule.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8950, Single Molecule Spectroscopy and Superresolution Imaging VII, 895008 (4 March 2014); doi: 10.1117/12.2035236
Show Author Affiliations
Siddharth Ghosh, International Max Planck Research School for Physics of Biological and Complex Systems (Germany)
Georg-August-Univ. Göttingen (Germany)
Manoj Kumbhakar, Bhabha Atomic Research Ctr. (India)
Mitja Platen, International Max Planck Research School for Physics of Biological and Complex Systems (Germany)
Georg-August-Univ. Göttingen (Germany)
Ingo Gregor, Georg-August-Univ. Göttingen (Germany)
Jörg Enderlein, Georg-August-Univ. Göttingen (Germany)


Published in SPIE Proceedings Vol. 8950:
Single Molecule Spectroscopy and Superresolution Imaging VII
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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