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Proceedings Paper

Cross-correlation analysis for live-cell image trajectory
Author(s): Chih-Ming Cheng; Yu-Fen Chang; Chien-ming Wu
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Paper Abstract

In cell motility, researchers are usually used fluorescence microscopy, confocal microscopy, or total internal reflection microscopy to track a fluorescent labeled particle and reveal the dynamic trajectory in living. Because all fluorescent dyes have cell toxicity, quantum dots and gold nanoparticles can influence the structures and physical properties of biomolecules which they have labeled, to develop another label-free image approach becomes an important issue. We present here a Fourier-based cross-correlation process to analyze images of adhering living cell, including cell motility and single vesicle trajectory. We treated adhering MG-63 cell with 66 nM Epidermal growth factor (EGF) and observed its dynamic effect on cell motility based on the velocity fields of consecutive cell images. We also used crosscorrelation to track single vesicles in living cells. We found that EGF could rapidly activate the motility of adhering MG- 63 cell, and the vesicle exhibits either directed or diffusive motion.

Paper Details

Date Published: 23 August 2013
PDF: 8 pages
Proc. SPIE 8911, International Symposium on Photoelectronic Detection and Imaging 2013: Micro/Nano Optical Imaging Technologies and Applications, 89110U (23 August 2013); doi: 10.1117/12.2034840
Show Author Affiliations
Chih-Ming Cheng, Industrial Technology Research Institute (Taiwan)
Yu-Fen Chang, Tao Shin Hospital (Taiwan)
Chien-ming Wu, National Tsing Hua Univ. (Taiwan)


Published in SPIE Proceedings Vol. 8911:
International Symposium on Photoelectronic Detection and Imaging 2013: Micro/Nano Optical Imaging Technologies and Applications
Min Gu; Xiaocong Yuan; Min Qiu, Editor(s)

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