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Proceedings Paper

Flow cytometry and other optical methods for characterization and quantification of phytoplankton in seawater
Author(s): Johannes W. Hofstraat; W. J. M. van Zeijl; J. C.H. Peeters; L. Peperzak; George B.J. Dubelaar
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Paper Abstract

New, optical, approaches to automatize and to accelerate the analysis of algae are discussed. Firstly, the application of flow cytometric analysis will be presented. This technique is based on classification of particles in flow by measuring several optical properties per individual particle. Characteristics of the Optical Plankton Analyser, a flow cytometer constructed specifically for the analysis of phytoplankton, will be presented. Two experiments that demonstrate the suitability of this instrument for the analysis of coastal phytoplankton will be presented. Subsequently, the applicability of flow cytometry and other optical approaches to the measurement of phytoplankton, in particular for monitoring purposes, will be discussed. Microscopy, as well as in-situ measurement and optical remote sensing are approaches that each offer specific advantages for the determination of phytoplankton. An optimal and integrated picture of the development of the distribution of phytoplankton species in space and time can be obtained by the combined application of these techniques.

Paper Details

Date Published: 1 August 1990
PDF: 17 pages
Proc. SPIE 1269, Environment and Pollution Measurement Sensors and Systems, (1 August 1990); doi: 10.1117/12.20348
Show Author Affiliations
Johannes W. Hofstraat, Ministry of Transport and Public Works (Netherlands)
W. J. M. van Zeijl, Ministry of Transport and Public Works (Netherlands)
J. C.H. Peeters, Ministry of Transport and Public Works (Netherlands)
L. Peperzak, Ministry of Transport and Public Works (Netherlands)
George B.J. Dubelaar, Institute of Applied Radiobiology and Immunology (Netherlands)

Published in SPIE Proceedings Vol. 1269:
Environment and Pollution Measurement Sensors and Systems
Hans Ole Nielsen, Editor(s)

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