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Proceedings Paper

PDT-induced apoptosis in arterial smooth muscles cells
Author(s): Isaac Nyamekye; R. Renick; C. Gilbert; Jean R. McEwan; G. Evan; Christopher C. R. Bishop; Stephen G. Bown
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Paper Abstract

PDT kills smooth muscle cells (SMC) in vivo and thus prevents intimal hyperplasia after angioplasty. It causes little inflammation and structural integrity of the artery is not compromised. We have studied the process of the SMC death in vitro. Cultured rat SMC (cell line sv40 ATCC) were sensitized with aluminum disulphonated phthalocyanine (AlS2Pc), and then irradiated with 675 nm laser light (2.5 J/cm2). Controls were studied using only sensitizer or laser for treatment. The cells were incubated and the dying process observed with a time lapse video and microscope system. PDT caused a characteristic pattern of death. Cells lost contact with neighbors, shrank, and showed hyperactivity and membrane ruffling. The cells imploded into active and condensed membrane bound vesicles which were terminally reduced to residual bodies. These are the morphological changes of apoptosis. The control cells which were given AlS2Pc alone or laser alone showed no death. PDT induced cultured arterial SMC death by apoptosis rather than necrosis. An apoptotic mechanism of cell death in vivo would explain the relative lack of inflammation and local tissue destruction in the face of massive death.

Paper Details

Date Published: 1 March 1995
PDF: 7 pages
Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); doi: 10.1117/12.203419
Show Author Affiliations
Isaac Nyamekye, The Rayne Institute/Univ. College London Medical School and Imperial Cancer Research Fund (United Kingdom)
R. Renick, Univ. College London Medical School (United Kingdom)
C. Gilbert, Imperial Cancer Research Fund (United Kingdom)
Jean R. McEwan, Univ. College London Medical School (United Kingdom)
G. Evan, Imperial Cancer Research Fund (United Kingdom)
Christopher C. R. Bishop, Imperial Cancer Research Fund (United Kingdom)
Stephen G. Bown, The Rayne Institute/Univ. College London Medical School (United Kingdom)


Published in SPIE Proceedings Vol. 2371:
5th International Photodynamic Association Biennial Meeting
Denis A. Cortese, Editor(s)

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