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Proceedings Paper

Fluorescence detection of premalignant, malignant, and micrometastatic disease using hexylpyropheophorbide
Author(s): Kinya Furukawa; David H. Crean; Thomas S. Mang; Harubumi Kato; Thomas J. Dougherty
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Paper Abstract

We investigated the in vivo fluorescence detection of premalignant and malignant lesions in carcinogen-induced tumors of the hamster buccal cheek pouch and micrometastases in rat lymph nodes using hexylpyropheophorbide-a (HPPH) at 0.5 mg/kg and He-Ne laser-induced fluorescence photometry. Time course studies of HPPH in hamster tumor model showed maximal relative fluorescence readings at 48 hours after i.p. injection in each stage (dysplasia: 0.35, papilloma: 0.58, squamous cell carcinoma: 1.04). Squamous cell carcinoma showed significantly greater fluorescence than papillomas and dysplasias at all time points (p < 0.01). Metastatic lymph nodes were significantly greater than normal lymph nodes at all time points (p < 0.01). Maximal fluorescence levels of normal lymph nodes were observed at 6 hours after i.v. injection. Metastatic lymph nodes still showed high fluorescence levels at 72 hours. Micrometastases showed fluorescence levels between the levels of metastatic and normal lymph nodes (normal: 0.40 < micrometastasis: 0.62 < metastasis: 0.75 at 48 hours after i.v. injection). The results demonstrated good correlation between fluorescence levels and histopathological developments at all time points. Therefore, HPPH may be a promising fluorophore for the detection of developing malignancies and occult disease.

Paper Details

Date Published: 1 March 1995
PDF: 5 pages
Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); doi: 10.1117/12.203403
Show Author Affiliations
Kinya Furukawa, Roswell Park Cancer Institute (United States)
Tokyo Medical College (Japan)
David H. Crean, Ontario Cancer Institute (Canada)
Thomas S. Mang, Roswell Park Cancer Institute (United States)
Harubumi Kato, Tokyo Medical College (Japan)
Thomas J. Dougherty, Roswell Park Cancer Institute (United States)

Published in SPIE Proceedings Vol. 2371:
5th International Photodynamic Association Biennial Meeting
Denis A. Cortese, Editor(s)

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