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Proceedings Paper

Laser measurements of bacterial endospore destruction from shock waves
Author(s): Petros P. Lappas; A. Daniel McCartt; Sean D. Gates; Jay B. Jeffries; Ronald K. Hanson
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Paper Abstract

The effects of shock waves on bioaerosols containing endospores were measured by combined laser absorption and scattering. Experiments were conducted in the Stanford aerosol shock tube for post-shock temperatures ranging from 400 K to 1100 K. Laser intensity measurements through the test section of the shock tube at wavelengths of 266 and 665 nm provided real-time monitoring of the morphological changes (includes changes in shape, structure and optical properties) in the endospores. Scatter of the visible light measured the integrity of endospore structure, while absorption of the UV light provided a measure of biochemicals released when endospores ruptured. For post-shock temperatures above 750 K the structural breakdown of Bacillus atrophaeus (BA) endospores was observed. A simple theoretical model using laser extinction is presented for determining the fraction of endospores that are ruptured by the shock waves. In addition, mechanisms of endospore mortality preceding their disintegration due to shock waves are discussed.

Paper Details

Date Published: 7 December 2013
PDF: 10 pages
Proc. SPIE 8923, Micro/Nano Materials, Devices, and Systems, 89231C (7 December 2013); doi: 10.1117/12.2033936
Show Author Affiliations
Petros P. Lappas, RMIT Univ. (Australia)
A. Daniel McCartt, Stanford Univ. (United States)
Sean D. Gates, Stanford Univ. (United States)
Jay B. Jeffries, Stanford Univ. (United States)
Ronald K. Hanson, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 8923:
Micro/Nano Materials, Devices, and Systems
James Friend; H. Hoe Tan, Editor(s)

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