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Proceedings Paper

Raman microspectroscopic study of oral buccal mucosa
Author(s): Isha Behl; Hitesh Mamgain; Atul Deshmukh; Lekha Kukreja; Arti R. Hole; C. Murali Krishna
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Paper Abstract

Oral cancer is the most common cancer among Indian males, with 5-year- survival-rates of less than 50%. Efficacy of Raman spectroscopic methods in non-invasive and objective diagnosis of oral cancers and confounding factors has already been demonstrated. The present Raman microspectroscopic study was undertaken for in-depth and site-specific analysis of normal and tumor tissues. 10 normal and 10 tumors unstained sections from 20 tissues were accrued. Raman data of 160 x 60 μm and 140 x 140 μm in normal and tumor sections, respectively, were acquired using WITec alpha 300R equipped with 532 nm laser, 50X objective and 600 gr/mm grating. Spectral data were corrected for CCDresponse, background. First-derivitized and vector-normalized data were then subjected to K-mean cluster analysis to generate Raman maps and correlated with their respective histopathology. In normal sections, stratification among epithelial layers i.e. basal, intermediate, superficial was observed. Tumor, stromal and inflammatory regions were identified in case of tumor section. Extracted spectra of the pathologically annotated regions were subjected to Principal component analysis. Findings suggest that all three layers of normal epithelium can be differentiated against tumor cells. In epithelium, basal and superficial layers can be separated while intermediate layer show misclassifications. In tumors, discrimination of inflammatory regions from tumor cells and tumor-stroma regions were observed. Finding of the study indicate Raman mapping can lead to molecular level insights of normal and pathological states.

Paper Details

Date Published: 17 March 2014
PDF: 7 pages
Proc. SPIE 8940, Optical Biopsy XII, 89400D (17 March 2014); doi: 10.1117/12.2033933
Show Author Affiliations
Isha Behl, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Hitesh Mamgain, WITec GmbH (Germany)
Atul Deshmukh, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Lekha Kukreja, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Arti R. Hole, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
C. Murali Krishna, Advanced Ctr. for Treatment, Research & Education in Cancer (India)

Published in SPIE Proceedings Vol. 8940:
Optical Biopsy XII
Robert R. Alfano; Stavros G. Demos, Editor(s)

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